MURRAY STATE UNIVERSITY

GRANT PROGRAM

GRANT PROGRAM

OCCUPATIONAL SAFETY AND HEALTH PROGRAM IMPROVEMENT

MURRAY STATE UNIVERSITY STUDENT SUPPORT SERVICES

AWARD PURPOSE.WORC PURPOSE: IN ALIGNMENT WITH THE JUSTICE40 INITIATIVE, THE PURPOSE OF THE WORC INITIATIVE GRANTS IS TO CREATE ECONOMIC MOBILITY, ADDRESS HISTORIC INEQUITIES FOR MARGINALIZED COMMUNITIES OF COLOR, RURAL AREAS, AND OTHER UNDERSERVED AND UNDERREPRESENTED COMMUNITIES, AND PRODUCE HIGH-QUALITY EMPLOYMENT OUTCOMES FOR WORKERS WHO LIVE OR WORK IN THE APPALACHIAN, DELTA, AND NORTHERN BORDER REGIONS, ENABLING THEM TO REMAIN AND THRIVE IN THESE COMMUNITIES. PROJECT TITLE: CYBERSECURITY ANALYST WORKFORCE DEVELOPMENT IN THE WEST KENTUCKY DRA REGIONGRANTEE REGION: DRADELIVERABLES EXPECTED OUTCOMES.TOTAL PARTICIPANTS: 64PARTICIPANTS OBTAINING NEW OR ENHANCED EMPLOYMENT: 64INTENDED BENEFICIARIES.TARGET POPULATION: INCUMBENT WORKERS, NEW ENTRANTS TO THE WORKFORCE, INCLUDING LAPSE IN EMPLOYMENT RETURNING MILITARY WOMEN RE-ENTERING THE WORKFORCE INCUMBENT WORKERS RECENT HIGH SCHOOL GRADUATES.AREAS TO BE SERVED: WEST KENTUCKY COUNTIES OF BALLARD, CALDWELL, CALLOWAY, CHRISTIAN, CRITTENDEN, FULTON, GRAVES, HENDERSON, HOPKINS, LIVINGSTON, LYON, MCCRACKEN, MCLEAN, MARSHALL, MUHLENBERG, TODD, TRIGG, UNION, AND WEBSTER.ACTIVITIES TO BE PERFORMED.SUMMARY OF PROJECT: MURRAY STATE UNIVERSITY CYBER EDUCATION AND RESEARCH CENTER WILL DEVELOP AND IMPLEMENT A WORKFORCE DEVELOPMENT CERTIFICATE PROGRAM TO EDUCATE AND TRAIN TO BECOME CYBERSECURITY ANALYSTS. THE TRAINING PROGRAM WILL PROVIDE A ONE-YEAR CYBERSECURITY CERTIFICATE TO 64 PARTICIPANTS WITH MULTIPLE INDUSTRY CERTIFICATIONS, CULMINATING WITH AN APPRENTICESHIP OPPORTUNITY. THIS PROJECT WILL PROVIDE FOCUSED, COMPREHENSIVE EDUCATION AND TRAINING TO INCUMBENT WORKERS AND NEW WORKFORCE ENTRANTS, TARGETING THOSE FROM MARGINALIZED COMMUNITIES WISHING TO PURSUE A CYBER ANALYST CAREER AND EQUIP INDIVIDUALS WITH THE NECESSARY SKILLS AND KNOWLEDGE TO DETECT, RESPOND TO, AND PREVENT CYBER-ATTACKS EFFECTIVELY. A PROGRAM FOCUS IS TO INCREASE CYBERSECURITY EMPLOYMENT OPPORTUNITIES FOR MARGINALIZED POPULATIONS WITHIN THE KENTUCKY DELTA REGION AND ULTIMATELY DEVELOP A SUSTAINABLE PIPELINE OF SKILLED CYBERSECURITY ANALYSTS TO MEET THE GROWING DEMAND IN THE CYBERSECURITY INDUSTRY. SUBRECIPIENT ACTIVITIES.THE RECIPIENT DOES NOT INTEND TO SUBAWARD FUNDS.

NURSE EDUCATION, PRACTICE, QUALITY AND RETENTION SIMULATION EDUCATION TRAINING PROGRAM

MURRAY STATE UNIVERSITY TALENT SEARCH WEST

MURRAY STATE UNIVERSITY TALENT SEARCH EAST

THE MURRAY STATE UNIVERSITY POLICE DEPARTMENT PROPOSES TO IMPLEMENT THE MURRAY STATE UNIVERSITY COMMUNICATIONS AND SECURITY CAMERA SYSTEMS ENHANCEMENT PROGRAM. THE PURPOSE IS THE REPLACEMENT OF THE MURRAY STATE POLICE DEPARTMENTS RADIO COMMUNICATIONS SYSTEM AS WELL AS THE REPLACEMENT AND ENHANCEMENT OF SECURITY CAMERA SOFTWARE AND HARDWARE ON MURRAY STATES MAIN CAMPUS, IN ADDITION TO ANY INFRASTRUCTURE, SUCH AS FIBER AND/OR ELECTRIC, THAT MAY BE NECESSARY FOR THE OPERATION OF NEW EQUIPMENT. PROJECT ACTIVITIES FOR THE COMMUNICATIONS CENTER INCLUDE REPLACING THE COMMUNICATION CENTERS MAIN RADIO CONSOLE, PORTABLE RADIOS FOR EACH OFFICER, AND MOBILE RADIOS FOR EACH PATROL VEHICLE. PROJECT ACTIVITIES FOR THE SECURITY CAMERA SYSTEM INCLUDE REPLACING AND IMPLEMENTING CAMERAS ACROSS CAMPUS, OBTAINING NEW SOFTWARE, AND ADDING ADDITIONAL INFRASTRUCTURE. EXPECTED OUTCOMES FOR COMMUNICATIONS INCLUDE SEAMLESS, UNINTERRUPTED COMMUNICATION BETWEEN DISPATCH AND OFFICERS, THE ABILITY TO COMMUNICATE TO LOCAL AGENCIES, ALONG WITH ENHANCING OUR ABILITIES TO SERVE AS THE EMERGENCY OPERATIONS CENTER FOR CALLOWAY COUNTY, KENTUCKY. EXPECTED OUTCOMES FOR THE SECURITY CAMERA SYSTEM INCLUDE IMPROVING COVERAGE, CRIME PREVENTION, INVESTIGATIONS, AND THE ABILITY TO MONITOR EVENTS IN REAL TIME ON MURRAY STATES MAIN CAMPUS. THESE COMBINED IMPROVEMENTS WILL BENEFIT THE FACULTY, STAFF, AND STUDENTS OF MURRAY STATE UNIVERSITY, AS WELL AS THE RESIDENTS OF CALLOWAY COUNTY, KENTUCKY.

DELTA HEALTH CARE SERVICES GRANT - CONACT SEC 379G

UPWARD BOUND MATH AND SCIENCE I

UPWARD BOUND CHRISTIAN COUNTY

UPWARD BOUND TRI STATE

UPWARD BOUND HENDERSON COUNTY

MURRAY STATE UNIVERSITY'S RONALD E. MCNAIR BACCALAUREATE ACHIEVEMENT PROGRAM

INTEINS: EXPANDING BIOLOGICAL ROLES AND BIOTECHNOLOGICAL APPLICATIONS - PROJECT SUMMARY/ABSTACT THE LONG-TERM GOALS OF THIS APPLICATION ARE TO BETTER UNDERSTAND THE BIOLOGICAL IMPORTANCE OF INTEINS AS POST- TRANSLATIONAL REGULATORY ELEMENTS, THEIR EMERGING ROLE IN PATHOGEN STRESS RESPONSE, AND TO HARNESS THE POWER OF THEIR UNIQUE CHEMISTRY TO INVENT USEFUL TECHNOLOGIES FOR THE BIOMEDICAL RESEARCH COMMUNITY. INTEINS, OR INTERVENING PROTEINS, ARE SELF-CATALYTIC, MOBILE GENETIC ELEMENTS REMOVED FROM HOST GENES THROUGH PROTEIN SPLICING. FROM THE APPLIED PERSPECTIVE, THE ABILITY OF INTEINS TO SHUFFLE PEPTIDE BONDS IN HIGHLY SPECIFIC WAYS HAS PROVEN EXCEPTIONALLY USEFUL IN PROTEIN ENGINEERING, LEADING TO THE DEVELOPMENT OF NUMEROUS TECHNOLOGIES. WHILE INTEIN APPLICATIONS HAVE DOMINATED THEIR INVESTIGATION, AND NEW INTEIN-BASED TECHNOLOGIES ARE DEVELOPED FREQUENTLY, THE BIOLOGICAL IMPORTANCE OF INTEINS IN NATURE IS POORLY UNDERSTOOD. INTEINS ARE ABUNDANT MOBILE GENETIC ELEMENTS IN THE MICROBIAL WORLD, FOUND IN APPROXIMATELY ONE-HALF OF ARCHAEA AND ONE-QUARTER OF BACTERIA. CONTRARY TO LONG-STANDING ASSUMPTION THAT INTEINS ARE MOLECULAR PARASITES, MOUNTING RECENT EVIDENCE SUGGESTS THAT SOME INTEIN-CONTAINING PROTEINS HAVE EVOLVED TO COUPLE SPLICING, AND THUS HOST PROTEIN ACTIVATION, TO ENVIRONMENTAL SIGNALS. THIS REPRESENTS A NOVEL AND POTENTIALLY WIDESPREAD FORM OF POST-TRANSLATIONAL REGULATION. FURTHER, GIVEN INTEINS ARE ABSENT IN HUMANS AND LOCATED WITHIN ESSENTIAL GENES OF SEVERAL PATHOGENS, UNDERSTANDING THE ENVIRONMENTAL FACTORS THAT REGULATE PROTEIN SPLICING INFORM POSSIBLE ANTIMICROBIAL DEVELOPMENT. WE PROPOSE THE FOLLOWING TWO AIMS, BUILDING UPON RECENT DISCOVERIES, AS WELL AS EXPANDING INTO NEW ARENAS. AIM 1 WILL FOR THE FIRST TIME INVESTIGATE THE ROLE INTEINS PLAY AS NASCENT CHAINS, BROADLY DETERMINING WHETHER SPLICING IS POSSIBLE PRIOR TO RELEASE FROM THE RIBOSOME, AS WELL AS EXAMINING POSSIBLE STRESS RESPONSE STRATEGIES OF BACTERIAL AND FUNGAL PATHOGENS. AIM 2 SEEKS TO DEVELOP TWO NOVEL INTEIN-BASED TECHNOLOGIES. FIRST, A GENERAL STRATEGY TO IMPROVE EXPRESSION OF MISFOLDING-PRONE PROTEINS IN BACTERIA AND SECOND, A ZINC- CONTROLLED SELF-REMOVING PROTEIN PURIFICATION TAG FOR USE IN BOTH BACTERIAL AND MAMMALIAN SYSTEMS. THROUGH THESE AIMS, THIS WORK WILL ENHANCE OUR UNDERSTANDING OF THE ROLES THESE EXCITING AND UNDERSTUDIED ELEMENTS PLAY IN NATURE, THEIR ROLE IN PATHOGEN STRESS RESPONSE, AND WILL LEAD TO NEW INTEIN-BASED TECHNOLOGIES FOR PROTEIN ENGINEERING.

THE PROJECT GOAL IS TO DEVELOP INFORMATION THAT DOE, CONTRACTORS, AND THE PUBLIC CAN UTILIZE IN DECISION-MAKING FOR THE ENVIRONMENTAL CLEANUP, BENEFICIAL REUSE, AND PUBLIC OUTREACH AT THE PADUCAH SITE.

THE VETERINARY AND ANIMAL SCIENCE TRAINING FOR TEACHERS (VASTT) PROJECT IS DESIGNED TO ADDRESS THE ANIMAL HEALTH AND PRODUCTION AND ANIMAL PRODUCTS PRIORITY AREA OF THE AFRI FARM BILL. THE PRIMARY OBJECTIVE OF THIS PROJECT IS TO PROVIDE PROFESSIONAL DEVELOPMENT IN ANIMAL SCIENCE AND VETERINARY SCIENCE AND ALIGNED RESOURCES TO 120 AGRICULTURE TEACHERS. THIS WILL BE ACCOMPLISHED THROUGH PROVIDING EXPERIENTIAL, INNOVATIVE TRAINING (INCLUDING TOURS OF LOCAL VETERINARY FACILITIES) TOAGRICULTURE TEACHERS. TEACHERS WILL IN TURN USE THE RESOURCES GIVEN TO THEM AT THEIR TRAINING SESSIONS (I.E., COMMERCIALLY-DEVELOPED TEXTBOOKS, DIGITAL CONTENT PRESENTATIONS, AND LABORATORY INSTRUCTION WORKBOOKS, LABORATORY TEACHING SUPPLY KITS, AND DIGITAL COPIES OF PROJECT TEAM-DEVELOPED MATERIALS SHARED VIA A GOOGLE DRIVE FOLDER) TO FACILITATE INSTRUCTION FOR THEIR STUDENTS. TRAINING SESSIONS (ONE ANNUALLY) WILL BEGIN IN THE SUMMER 2026 SEMESTER AND CONCLUDE IN THE SUMMER 2029 SEMESTER. EVERY PARTICIPATING AGRICULTURE TEACHER WILL BE PROVIDED WITH RESOURCES TO TAKE BACK TO THEIRSCHOOL. IT IS ANTICIPATED THAT 6,000 STUDENTS WILL BE INDIRECTLY IMPACTED BY THIS PROJECT. THE SECONDARY OBJECTIVE OF THIS PROJECT IS TO SUPPORT FACULTY-LED, COURSE CREDIT-BEARING, STIPEND-FUNDED MENTORSHIP OPPORTUNITIES FOR 16 UNDERGRADUATE AGRICULTURAL EDUCATION STUDENTS. EXHAUSTIVE, MULTI-FACETED PROJECT EVALUATION METHODS WILL BE EMPLOYED BY SELECTED PROJECT TEAM MEMBERS AND THE EXTERNAL EVALUATOR. SEVERAL SCHOLARLY PRESENTATIONS AND PUBLICATIONS WILL BE GENERATED BY THIS PROJECT. AMURRAY STATE UNIVERSITY-HOSTED PROJECT INFORMATION WEBSITE WILL BE DEVELOPED AND REGULARLY MAINTAINED BY THE PROJECT TEAM.

CAMPUS BASED-FWS

CAMPUS BASED-FWS

2025-2026 TEACH BASE RECORD

PURPOSE BECAUSE INVASIVE CARP FEED LOW ON THE AQUATIC FOOD WEB, THEY ARE LIKELY TO IMPACT MANY NATIVE FISH SPECIES IN KENTUCKY LAKE AND LAKE BARKLEY THUS, IT IS IMPORTANT THAT WE STUDY NOT ONLY THE INVASIVE CARP BUT ALSO THE NATIVE FISH WHICH ARE LIKELY TO BE INFLUENCED BY THOSE CARP WE PROPOSE TO USE ACOUSTIC TAGS TO TRACK THE MOVEMENT OF BOTH INVASIVE CARP AND NATIVE SPECIES THROUGHOUT THESE RESERVOIRS, INCLUDING THE PASSAGE OF FISH ACROSS THE DAMS OF BOTH RESERVOIRS ALSO, NATIVE GIZZARD SHAD AND THREADFIN SHAD ARE CRITICAL FORAGE FISH IN THESE RESERVOIRS AND ARE EXPECTED TO COMPETE DIRECTLY WITH INVASIVE CARP THUS, WE ALSO PROPOSE TO STUDY THE POPULATION DYNAMICS OF THE NATIVE SHAD SPECIES SO THAT WE CAN CLOSELY MONITOR HOW THESE FISH ARE AFFECTED BY INVASIVE CARP ACTIVITIES TO BE PERFORMED ACOUSTIC TAGS WILL BE IMPLANTED IN INVASIVE CARP, PADDLEFISH, AND SMALLMOUTH BUFFALO SO THAT THESE FISH CAN BE TRACKED THROUGHOUT EACH RESERVOIR FISH MOVEMENT WILL BE MONITORED BOTH ON A LARGE SCALE VIA THE EXISTING NETWORK OF PASSIVE RECEIVERS, AND ALSO ON A SMALL SCALE BY MOAT MOUNTED TRACKING BOAT MOUNTED TRACKING WILL FOCUS ON 24 HOUR MOVEMENT PATTERNS AND MACROHABITAT USE OF THESE SPECIES SHAD SPECIES WILL BE PERIODICALLY SAMPLED BY A VARIETY OF GEARS TO COLLECT A REPRESENTATIVE SAMPLE OF THESE POPULATIONS ALL SHAD WILL BE MEASURED, AND A SUBSAMPLE WILL BE AGED IN EACH RESERVOIR DELIVERABLES AND EXPECTED OUTCOMES WE WILL PROVIDE FINAL REPORTS CONCERNING THE MOVEMENT AND DAM CROSSING OF BOTH INVASIVE CARP AND THE NATIVE SPECIES WE WILL ALSO PROVIDE FINAL REPORTS CONCERNING THE POPULATION CHARACTERISTICS OF BOTH SHAD SPECIES IN KENTUCKY LAKE AND LAKE BARKLEY INTENDED BENEFICIARIES FISHING AND RECREATION WITHIN THESE 2 RESERVOIRS ARE CRITICAL COMPONENTS OF THE CULTURE AND ECONOMY OF WESTERN KENTUCKY THE INVASIVE CARP THREATEN TO DISRUPT THE ENTIRE AQUATIC COMMUNITY OF THESE SYSTEMS, AND THEY ALSO CREATE HAZARDS FOR RECREATIONAL BOATERS

CAMPUS BASED-FSEOG

MURRAY STATE UNIVERSITY STUDENT SUPPORT SERVICES

CAMPUS BASED-FSEOG

CVM VET-LRN VETERINARY DIAGNOSTIC LABORATORY PROGRAM

RII TRACK-4:@NASA: METHANE DYNAMICS DESCRIBED THROUGH THE FUSION OF SITE AND SATELLITE DATA IN BOTTOMLAND HARDWOOD FORESTED AND NON-FORESTED WETLANDS -THIS RESEARCH INFRASTRUCTURE IMPROVEMENT TRACK-4 EPSCOR RESEARCH FELLOWS (RII TRACK-4) PROJECT WILL PROVIDE A FELLOWSHIP TO AN ASSOCIATE PROFESSOR AND TRAINING FOR A GRADUATE STUDENT AT MURRAY STATE UNIVERSITY. THIS WORK WILL BE CONDUCTED IN COLLABORATION WITH RESEARCHERS AT NASA AMES RESEARCH CENTER. THROUGH THE FELLOWSHIP, THE PI AIMS TO STUDY THE EFFECTS OF CHANGING CLIMATE ON GREENHOUSE GAS (GHG) EMISSIONS IN WETLAND ECOSYSTEMS. THE MAIN GOAL OF THIS FELLOWSHIP IS TO BETTER UNDERSTAND THE DYNAMICS OF GHGS WITHIN WETLAND ECOSYSTEMS BY MEASURING GHG FLUXES IN MINERAL SOIL FORESTED WETLANDS THAT ARE NOT WELL STUDIED. THE PROJECT WILL AIM TO IMPROVE THE DETECTION OF GHG EMISSIONS FROM WETLANDS USING SATELLITES, REDUCE UNCERTAINTIES IN THE GHG FLUXES ACROSS LARGER SPATIAL EXTENTS, AND VALIDATE SATELLITE-BASED GHG EMISSION DATA. THE PROJECT DESIGN ALLOWS FOR THE QUANTIFICATION OF GHG FLUXES FROM ALL MAJOR POOLS (I.E., SOILS, STEMS, KNEES), AND FOR THE INVESTIGATION OF SOIL-VEGETATION INTERACTIONS. THE FELLOWSHIP WILL STRENGTHEN THE PRINCIPAL INVESTIGATOR?S ABILITY TO ATTRACT DIVERSE STUDENTS (WOMEN, FIRST-GENERATION, AND MINORITY STUDENTS) TO THE FIELD AND PROVIDE THEM WITH OPPORTUNITIES TO DEVELOP THE RESEARCH SKILLS THAT CAN LEAD THEM TO SUCCESSFUL FUTURE CAREERS. IN ADDITION, THE FELLOWSHIP WILL HAVE A BROADER IMPACT ON THE JURISDICTION. FORESTED ECOSYSTEM CARBON FLUX DYNAMICS HAVE A TREMENDOUS IMPACT ON GLOBAL CLIMATE. IN THIS PROJECT, THE PI WILL EXTEND KNOWLEDGE OF CARBON DYNAMICS BY ESTABLISHING A NEW SITE FOR MEASURING GHGS FLUXES IN MINERAL SOIL FORESTED WETLANDS THAT ARE NOT WELL STUDIED. THE OVERARCHING OBJECTIVES ARE TO (1) IMPROVE UNDERSTANDING OF THE CONTROLS ON GHG FLUXES IN MINERAL SOIL WETLANDS, AND (2) TO BETTER UNDERSTAND THE EFFECTS OF LANDSCAPE POSITION AND FOREST COMPOSITION ON THE GHG FLUXES BETWEEN TERRESTRIAL ECOSYSTEMS AND THE ATMOSPHERE. THE PROPOSED METHODOLOGY IS DESIGNED TO USE BOTH EMPIRICAL MEASUREMENTS TO IDENTIFY HOT SPOTS AND HOT MOMENTS OF GHG FLUXES IN THESE SYSTEMS AND IMPROVE REGIONAL GHG PREDICTIONS. THE STRATEGY OF LINKING THE FIELD CAMPAIGN WITH REMOTE SENSING ACTIVITIES IS AIMED AT PROVIDING NEW INSIGHTS INTO THE MAPPING OF THE CONTROLS OF MINERAL SOIL WETLAND CH4 FLUXES. NASA AMES RESEARCH CENTER (ARC) FACILITIES WILL BE ESSENTIAL FOR THE PROCESSING AND ANALYSIS OF THE SATELLITE IMAGERY NEEDED TO ACCOMPLISH THE TASK OF FELLOWSHIP. FURTHERMORE, HOST COLLABORATORS WILL OFFER EXPERIENCE IN METHANE MODELING USING REMOTE SENSING, FLUX TOWER, AND FIELD OBSERVATIONS AS INPUT VARIABLES. THE PI WILL BE ADVISED IN THE RESEARCH ASSOCIATED WITH AQUATIC SYSTEM SATELLITE AND AIRBORNE REMOTE SENSING EXPERTISE AS WELL AS ASSOCIATED ALIGNED AQUATIC FIELD OBSERVATIONS TO ASSIST IN ACCOMPLISHING THE PROPOSED TASKS. THE INFORMATION GAINED FROM THIS FELLOWSHIP WILL PROVIDE INSIGHTS ABOUT DRIVERS OF TREE STEM AND BALD CYPRESS KNEES GHG EMISSIONS. THIS AWARD REFLECTS NSF'S STATUTORY MISSION AND HAS BEEN DEEMED WORTHY OF SUPPORT THROUGH EVALUATION USING THE FOUNDATION'S INTELLECTUAL MERIT AND BROADER IMPACTS REVIEW CRITERIA.- SUBAWARDS ARE NOT PLANNED FOR THIS AWARD.

COLLABORATIVE RESEARCH: CHANGES IN LANDSCAPE, LABOR AND LIVELIHOOD IN LONG CHRONOLOGICAL CONTEXT

ELUCIDATING THE MECHANISMS OF GLIS3-REGULATED DEVELOPMENT IN THE PANCREAS AND KIDNEY - PROJECT SUMMARY EMBRYONIC DEVELOPMENT IS ORCHESTRATED THROUGH COMPLEX NETWORKS OF GENES THAT SPECIFY CELL FATES AND CHOREOGRAPH THE MIGRATION AND REORGANIZATION OF CELLS INTO TISSUES. MUTATIONS WITHIN DEVELOPMENTAL GENES CAN BOTH GIVE RISE TO BIRTH DEFECTS AND INCREASE SUSCEPTIBILITY TO DISEASES LATER IN LIFE. THE TRANSCRIPTION FACTOR, GLI-SIMILAR 3 (GLIS3) HAS BEEN FOUND TO BE IMPORTANT DURING EMBRYOLOGICAL DEVELOPMENT AND MUTATIONS WITHIN THE GLIS3 LOCUS HAVE BEEN IMPLICATED IN THE DEVELOPMENT OF SEVERAL DISEASES INCLUDING DIABETES, ALZHEIMER’S DISEASE, AND CANCER. CURRENTLY, LITTLE IS KNOWN ABOUT THE SPECIFIC ROLES GLIS3 PLAYS DURING DEVELOPMENT OR HOW DIVERGENCE FROM ITS NORMAL FUNCTION PROMOTES DISEASE AT THE MOLECULAR LEVEL. IN THIS PROPOSAL, EXPERIMENTS ARE DESCRIBED INTENDED TO CHARACTERIZE PANCREATIC AND RENAL DEVELOPMENT IN THE GLIS3EX4DEL/EX4DEL MUTANT ZEBRAFISH LINE THAT LACKS FUNCTIONAL GLIS3 EXPRESSION. IN SPECIFIC AIM 1, WE WILL CHARACTERIZE PANCREATIC DEVELOPMENT IN GLIS3 KNOCKOUT FISH TO DETERMINE WHAT ROLE GLIS3 PLAYS IN DIRECTING DEVELOPMENT OF THE PANCREATIC DUCTS AND THE SPECIFICATION OF SECONDARY ISLETS THAT EMERGE FROM THE DUCTAL EPITHELIUM. THE EXPERIMENTS DESCRIBED IN SPECIFIC AIM 2 ARE DESIGNED TO EXAMINE THE MECHANISMS BY WHICH LOSS OF GLIS3 EXPRESSION RESULTS IN CYSTIC KIDNEYS. PARTICULAR EMPHASIS WILL BE PLACED ON CHARACTERIZING THE ASSOCIATION BETWEEN GLIS3 AND THE MECHANO-SENSORY ORGANELLE KNOWN AS THE PRIMARY CILIUM. FINALLY, IN SPECIFIC AIM 3, PLANS ARE DESCRIBED TO GAIN A BETTER UNDERSTANDING OF THE SPECIFIC CELL TYPES THAT EXPRESS GLIS3 DURING DEVELOPMENT AND HOW LOSS OF GLIS3 EXPRESSION ALTERS GENE EXPRESSION IN THE DEVELOPING ZEBRAFISH BY CONDUCTING SCRNA- SEQ ANALYSIS. THE RESEARCH DESCRIBED IN THIS PROPOSAL WILL BE CONDUCTED AT A REGIONAL COMPREHENSIVE UNIVERSITY THAT SERVES A PREDOMINATELY RURAL, ECONOMICALLY UNDERDEVELOPED COMMUNITY FOR WHOM ACCESS TO SCIENTIFIC RESEARCH IS TRADITIONALLY RESTRICTED. THE RESEARCH WILL BE COMPLETED WITH EXTENSIVE SUPPORT FROM UNDERGRADUATE RESEARCHERS WHO WILL GAIN THE OPPORTUNITY TO CO-AUTHOR MANUSCRIPTS AND PRESENT FINDINGS AT LOCAL, STATE, AND NATIONAL MEETINGS. BROADLY, THE COMPLETION OF THE STUDIES OUTLINED IN THIS PROPOSAL WILL INCREASE OUR UNDERSTANDING OF HOW GLIS3 SPECIFIES CELL FATES DURING DEVELOPMENT AND MAY HELP SHED LIGHT ON HOW GLIS3 MUTATIONS LEAD TO DISEASES IN HUMANS SUCH AS DIABETES MELLITUS AND POLYCYSTIC KIDNEY DISEASE. FURTHERMORE, A GREATER UNDERSTANDING OF THE MECHANISMS THAT REGULATE PANCREATIC AND RENAL DEVELOPMENT IS LIKELY TO BE ACHIEVED INCLUDING A MORE THOROUGH KNOWLEDGE OF THE TRANSCRIPTION FACTOR NETWORKS THAT DIRECT CELL FATES AND TISSUE ORGANIZATION. ULTIMATELY, COMPLETION OF THE RESEARCH DESCRIBED MAY LEAD TO THE ESTABLISHMENT OF A COMPLEMENTARY ZEBRAFISH MODEL FOR THE STUDY OF HUMAN DISEASE.

RUI: CHE-DRP (SYN): CAS: TEMPERATURE-CONTROLLED MECHANOSYNTHESIS AS A SUSTAINABLE SYNTHETIC PLATFORM FOR PREPARATION OF TRANSITION METAL COMPLEXES -WITH THE SUPPORT OF THE CHEMICAL SYNTHESIS PROGRAM IN THE DIVISION OF CHEMISTRY AND THE ESTABLISHED PROGRAM TO STIMULATE COMPETITIVE RESEARCH (EPSCOR), RACHEL J. ALLENBAUGH OF MURRAY STATE UNIVERSITY WILL STUDY SMALL MOLECULE MECHANOSYNTHESIS KINETICS UNDER TEMPERATURE-CONTROLLED CONDITIONS TO ENHANCE BASIC UNDERSTANDING OF MECHANOCHEMISTRY. MECHANOCHEMISTRY HAS THE POTENTIAL TO OPEN UP NEW, MORE SUSTAINABLE CHEMISTRY METHODS. TRADITIONAL CHEMICAL PROCESSES INVOLVE DISSOLVING STARTING MATERIALS IN A SOLVENT AND HEATING. THE SOLVENT USUALLY MUST BE REMOVED AND OFTEN BECOMES A HAZARDOUS WASTE. CONSEQUENTLY, NEW METHODS NOT INVOLVING SOLVENTS WOULD BE VERY DESIRABLE. MECHANOSYNTHESIS IS SUCH A TECHNIQUE THAT DOES NOT RELY ON SOLVENTS AND REPLACES THERMAL ENERGY DERIVED FROM HEATING WITH MECHANICAL IMPACTS. THIS TECHNIQUE ALSO TENDS TO IMPROVE YIELDS WHILE REDUCING REACTION TIME, THEREBY INCREASING THE EFFICIENCY AND LOWERING THE COST OF A PROCESS. DR. ALLENBAUGH AND HER GROUP WILL BE DEVELOPING METHODS FOR TEMPERATURE-CONTROLLED BALL-MILLING TO BETTER UNDERSTAND THE KINETICS AND MECHANISMS IN THE PRODUCTION OF COMMERCIALLY RELEVANT TRANSITION METAL COMPLEXES. IN THIS PROJECT, A DIVERSE GROUP OF UNDERGRADUATE AND M.S. STUDENTS WILL BE TRAINED IN SUSTAINABLE SYNTHESIS METHODS. HIGH SCHOOL STUDENTS WILL BE INTRODUCED TO SUSTAINABLE CHEMISTRY THROUGH CONTINUING EDUCATION ACTIVITIES WITH LOCAL HIGH SCHOOL TEACHERS. METHODS FOR INTEGRATING MECHANOSYNTHESIS AS A PART OF A COST-EFFECTIVE, SUSTAINABLE SYNTHETIC METHODOLOGY FOR PRIMARILY UNDERGRADUATE INSTITUTIONS WILL BE EXPLORED. DESPITE THE ADVANTAGE OF MECHANOSYNTHESIS, WIDESPREAD ADOPTION IS HINDERED BY THE ABSENCE OF SYSTEMATIC REACTION OPTIMIZATION METHODS AND THE LIMITED KINETIC UNDERSTANDING IN SMALL MOLECULE MECHANOSYNTHESIS. DR. ALLENBAUGH AND HER GROUP WILL ANALYZE MECHANOSYNTHESIS KINETICS FOR THE FORMATION OF A WIDE VARIETY OF METAL COMPLEXES, PALLADACYCLE CATALYSTS, AND PRE-CATALYSTS INCLUDING THE NAJERA II AND 2-AMINOBIPHENYL PALLADACYCLES. THROUGH THE DEVELOPMENT OF A TEMPERATURE-CONTROLLED BALL MILLING APPARATUS, MECHANOSYNTHETIC TEMPERATURE EFFECTS WILL BE EXPLORED. KINETIC MODELLING WILL ALLOW FOR A MORE THOROUGH UNDERSTANDING OF THE MECHANISMS OF MECHANOSYNTHESIS, RESULTING IN MORE RATIONAL METHODS FOR OPTIMIZING YIELDS AND REACTION TIMES. MORE BROADLY, DR. ALLENBAUGH?S RESEARCH SEEKS TO INTRODUCE A WIDER COMMUNITY TO ALTERNATIVE METHODS FOR IMPARTING ENERGY INTO CHEMICAL REACTIONS, AN APPROACH THAT INCLUDES MECHANOSYNTHESIS AND MICROWAVE CHEMISTRY. THESE AVENUES ARE LESS EXPLORED THAN METHODS UTILIZING DIRECT THERMAL OR LIGHT ENERGY TO DRIVE CHEMICAL REACTIONS AND HAVE THE POTENTIAL TO WIDEN THE PALETTE OF REACTION MODALITIES AVAILABLE TO CHEMISTS AND CHEMICAL ENGINEERS. THIS AWARD REFLECTS NSF'S STATUTORY MISSION AND HAS BEEN DEEMED WORTHY OF SUPPORT THROUGH EVALUATION USING THE FOUNDATION'S INTELLECTUAL MERIT AND BROADER IMPACTS REVIEW CRITERIA.- SUBAWARDS ARE NOT PLANNED FOR THIS AWARD.

PSYCHOTROPIC COMPOUNDS FROM WASTEWATER CONTAMINATION AFFECT NERVOUS SYSTEM DEVELOPMENT - ABSTRACT THE RECEIVING WATER BODIES THAT ARE SOURCES OF DRINKING WATER ARE CONTINUOUSLY CONTAMINATED WITH PSYCHOTROPIC DRUGS – THOSE THAT TARGET THE NERVOUS SYSTEM - THROUGH WASTEWATER TREATMENT FACILITY EFFLUENT. WITHOUT MECHANISMS TO REMOVE OR NEUTRALIZE THEM, THESE DRUGS CAN REACH THE DRINKING WATER, CROSS MATERNAL BIOLOGICAL BARRIERS, AND ALTER EMBRYONIC GENE EXPRESSION. IS IT NOT KNOWN HOW CHRONIC EXPOSURE TO THE COMPOSITE MIXTURE OF THESE DRUGS, INCLUDING ANTIDEPRESSANTS AND NARCOTICS, EVEN AT VERY LOW DOSES, MAY REPRESENT A RISK TO HUMAN HEALTH. PSYCHOTROPIC MEDICATIONS ARE AMONG THE MOST COMMONLY PRESCRIBED DRUGS IN THE U.S. TOGETHER WITH PSYCHOTROPIC ILLICIT DRUGS, A SIGNIFICANT PORTION OF THESE MEDICATIONS AND THEIR ACTIVE METABOLITES ARE DISCHARGED INTO THE RECEIVING WATER BODIES. WITH THE PREVALENCE OF DRUGS IN WASTEWATER EVER-INCREASING, SO DOES THE URGENCY OF UNDERSTANDING THE RISKS. OUR CENTRAL HYPOTHESIS IS THAT DEVELOPMENTAL EXPOSURE TO LOW LEVELS OF PSYCHOACTIVE DRUGS THROUGH CONTAMINATED WATER SOURCES AFFECTS GENE EXPRESSION IN THE DEVELOPING NERVOUS SYSTEM WITH BEHAVIORAL CONSEQUENCES. THIS PROPOSED BASIC BIOMEDICAL RESEARCH WILL EXPOSE DEVELOPING ZEBRAFISH TO AN ENVIRONMENTALLY-RELEVANT COMBINATION OF PSYCHOTROPIC COMPOUNDS TO EVALUATE THE EFFECTS OF THIS FORM OF EXPOSURE ON GENE EXPRESSION AND FUNCTION OF NERVOUS SYSTEM. QUANTITATIVE RT-PCR AND IN SITU HYBRIDIZATION WILL BE USED TO EXPLORE NERVOUS SYSTEM-SPECIFIC CHANGES IN GENE EXPRESSION FOLLOWING DRUG EXPOSURE. A BATTERY OF LARVAL BEHAVIORS RANGING FROM BASIC LOCOMOTION TO COGNITIVE TASKS WILL ASSESS THE FUNCTIONAL CONSEQUENCES OF DEVELOPMENTAL DRUG EXPOSURE. FINALLY, SENSITIVE BEHAVIORS WILL BE USED TO EVALUATE POTENTIAL SYNERGISM BETWEEN DRUGS. BY RAISING ZEBRAFISH IN DOSED WATER AS A PROXY FOR HUMAN PRENATAL EXPOSURE THROUGH CONTAMINATED WATER, THESE EXPERIMENTS WILL IDENTIFY SENSITIVE BEHAVIORS AND GENES THAT ARE EXPRESSED IN DEVELOPING NEURAL TISSUES. STRENGTHENING THE EVIDENCE THAT EXPOSURE TO SUBTHERAPEUTIC LEVELS OF THESE DRUGS HAS ADVERSE EFFECTS ON DEVELOPMENT MAY INFORM INTERVENTION TO REMEDIATION MEASURES TO REDUCE THE RISK TO HUMAN HEALTH.

NURSE ANESTHETIST TRAINEESHIPS

ALL NAHLN LEVEL 1, LEVEL 2 AND LEVEL 3 LABORATORIES WILL RECEIVE ANNUAL INFRASTRUCTURE SUPPORT FROM THE USDA/APHIS. THE PURPOSE OF THIS AGREEMENT IS TO ASSIST THE LABORATORY IN MEETING THE RESPONSIBILITIES REQUIRED OF THEIR DESIGNATED LEVEL AS OUTLINED IN THE 2024 AGREEMENT FOR PARTICIPATION IN THE NATIONAL ANIMAL HEALTH LABORATORY NETWORK (NAHLN). IN ADDITION, IT WILL SUPPORT AN OVERALL INCREASE IN NAHLN CAPACITY AND CAPABILITIES TO MEET THE NEEDS FACED DURING AN ADVERSE ANIMAL HEALTH EVENT CAUSED BY THE PRESENCE OF AN EMERGING DISEASE OR FOREIGN ANIMAL DISEASE. THIS WILL BE ACCOMPLISHED BY PROVIDING SUPPORT FOR; QUALITY MANAGEMENT SYSTEMS, LABORATORY INFORMATION MANAGEMENT SYSTEMS TO ELECTRONICALLY SUBMIT TESTING DATA ACCURATELY AND CONSISTENTLY, IMPROVING STAFFING EXPERTISE AND PROFICIENCY, AND ENHANCING EQUIPMENT THAT WILL DIRECTLY ENHANCE WORK PRODUCT, TEST OUTCOMES, ETC. THIS AGREEMENT WILL BENEFIT THE OVERALL NETWORK AS WELL AS THE AMERICAN POPULATION BY HELPING TO ENSURE THE RAPID RESPONSE TO AN ADVERSE ANIMAL HEALTH EVENT.

HPAI 12639521D0082 ORDER FOR TESTS 3.01, 3.02, 3.03

RII TRACK 4 ..NASA METHANE DYNAMICS DESCRIBED THROUGH THE FUSION OF SITE AND SATELLITE DATA IN BOTTOMLAND HARDWOOD FORESTED AND NON FORESTED WETLANDS

KENTUCKY AI LAB TESTING ATTN: CHARLENE LAMBERT. ALL TESTING SERVICES SHALL BE IN ACCORDANCE WITH ATTACHED SPECIFICATION

NATIONAL ANIMAL HEALTH LABORATORY NETWORK ORDER FOR HPAI TESTING.

BREATHITT VET CENTER KENTUCKY FY 09 NAHLN AI LAB TESTING ATTN:MARGIE THORSON. NEW BPA ALL TESTING SERVICES SHALL BE IN ACCORDANCE WITH SPECIFICATIONS

THE SHARED EXPERTISE AND RESOURCES WILL FORM THE BASIS OF A COOPERATIVE RESEARCH PROGRAM BETWEEN THE UNITED STATES GEOLOGICAL SURVEY USGS OHIOKENTUCKYINDIANA OKI WATER SCIENCE CENTER, LOCATED IN LOUISVILLE, KY, OTHER USGS OFFICES, AND MURRAY STATE UNIVERSITY MSU TO BENEFIT USGS AND MSU. THE COOPERATIVE RESEARCH PROGRAMS IN WATER RESOURCES AND OTHER RELATED AREAS MAY INCLUDE STUDENT INVOLVEMENT, OPPORTUNITIES FOR MSU TO PROVIDE TECHNICAL EXPERTISE AND THE SHARING OF MSU RESEARCH FACILITIES AND EQUIPMENT. MSU WILL MAKE AVAILABLE TO USGS FACILITIES, OFFICE SPACE, LIBRARY RESOURCES, AND LABORATORY AND FIELD EQUIPMENT. THE COOPERATIVE RESEARCH PROGRAM PRESENTS AN OPPORTUNITY FOR THE USGS AND MSU TO COLLABORATE IN THE GENERAL AREAS OF BIOLOGICALAND WATER RESOURCES.

NATIONAL ANIMAL HEALTH LABORATORY NETWORK ORDER FOR HPAI-AVIAN TESTING

55338 / 118419 HPAI TESTING DELIVERY ORDER FOR IDIQ CONTRACT 12639521D0082

MURRAY STATE UNIVERSITY FOR 1200 AGID AND 650 PCR SAMPLES FOR AI PROJECT. THIS IS TO INCREASE PO# AG-6395-B-09-0021

THE CLARKS RIVER NATIONAL WILDLIFE REFUGE (CRNWR) PROVIDES CRITICAL BOTTOMLAND HARDWOODWETLANDS FOR FOREST INTERIOR BIRD SPECIES, MANY OF WHICH ARE DESIGNATED AS SPECIES OF GREATESTCONSERVATION NEED (SGCN) IN KENTUCKY. WITH APPROXIMATELY 81% OF THE STATES BOTTOMLANDHARDWOODS LOST, UNDERSTANDING HOW FOREST MANAGEMENT PRACTICES IMPACT THESE BIRD COMMUNITIESIS ESSENTIAL FOR CONSERVATION PLANNING. WE AIM TO MONITOR BIRD SPECIES OCCURRENCE ON SITE USINGACOUSTIC RECORDERS SUCH THAT WE CAN EVALUATE THE EFFECTS OF CURRENT AND FUTURE FOREST MANAGEMENTACTIVITIES ON FOREST INTERIOR BIRDS AND DEVELOP HABITAT SUITABILITY MODELS FOR SGCN SPECIES. ABEFORE-AFTER CONTROL-IMPACT (BACI) STUDY DESIGN WILL BE USED TO ASSESS BIRD COMMUNITYCHANGES ACROSS 64 SYSTEMATICALLY PLACED SAMPLING LOCATIONS REPRESENTING BOTH MANAGED (N 19)AND CONTROL UNITS WITHIN CRNWR (N 45). ALTHOUGH THE CURRENT DATASET IS SKEWED TOWARDCONTROL UNITS, IT WILL ALSO SERVE AS BASELINE DATA FOR UNITS SCHEDULED FOR FUTURE MANAGEMENT,ENABLING FURTHER COMPARISONS OVER TIME. AUDIOMOTH BIOACOUSTIC RECORDERS WERE DEPLOYED IN2021 AND 2022 TO ESTABLISH PRE-TREATMENT BIRD COMMUNITY BASELINES. IN SPRING 2026, SURVEYS WILLBE CONDUCTED USING THE SAME PROTOCOL. COLLECTED AUDIO DATA WILL BE ANALYZED USING BIRDNETANALYZER, APPLYING AUTOMATED SPECIES IDENTIFICATION WITH GEOGRAPHIC AND SEASONAL FILTERS TOIMPROVE ACCURACY. A SUBSET OF RECORDINGS WILL BE MANUALLY REVIEWED TO VALIDATE RESULTS. MULTI-SPECIES OCCUPANCY MODELS (MSOM) WILL BE USED TO EVALUATE FOREST MANAGEMENT IMPACTS ONBIRD COMMUNITIES AT BOTH THE INDIVIDUAL SPECIES AND FUNCTIONAL GUILD LEVELS AND HABITAT SUITABILITYMODELS WILL BE COMPLETED FOR SGCN SPECIES. FINDINGS FROM THIS RESEARCH WILL DIRECTLY INFORMCRNWRS FOREST MANAGEMENT STRATEGIES, ENSURING THAT HABITAT RESTORATION EFFORTS SUPPORT LONGTERMCONSERVATION GOALS. ADDITIONALLY, RESULTS WILL PROVIDE GUIDANCE FOR SIMILAR MANAGEMENTPRACTICES AT GREEN RIVER NWR AND OTHER BOTTOMLAND HARDWOOD CONSERVATION AREAS.

ATTN: MPL CONTRACTING//LATISHA HOLLMAN//MPL-6197-20-33920//REQ # 73064//BREATHITT VETERINARY CENTER

GIS

NAHLN AMR/AST TESTING CLIN0001.03

NAHLN KENTUCKY AMR AST TESTING

56991/122640 BREATHITT VETERINARY CENTER. HPAI TESTING DELIVERY ORDER IDIQ: 12639521D0082 END USER: TARI MOODY 515-337-7928 IPP APPROVER: TARI MOODY

IGF::OT::IGF TUITION AND FEES

TUITION AND FEES